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Objective To explore the value of dynamic contrast -enhanced magnetic resonance imaging in differential diagnosis of benign and malignant ovarian tumors .Methods 50 patients with benign ovarian cancer were selected as the benign group ,and 50 patients with malignant ovarian cancer were selected as the malignant group .All patients underwent dynamic contrast-enhanced magnetic resonance imaging .Results The difference of the time intensity curve ( TIC) curve between the benign group and the malignant group was no statistically significant ( P<0.05),the benign group was mainly type Ⅰ curve,and the malignant group was dominated by type Ⅲ curve.The values of Ve,Kep and Ktrans in the benign ovarian tumors [(0.492 ±0.227),(0.163 ±0.081)min-1,(0.071 ± 0.034) min-1 ] were significantly lower than those in the malignant group [( 0.686 ±0.153 ), ( 0.411 ± 0.214)min-1,(0.282 ±0.110)min-1](P<0.05).The ROC curve under the area of Ve value,Kep value,Ktrans value to identify ovarian benign and malignant were 0.707,0.942,1.000;the best differential value of Ve ,Kep, Ktransfor ovarian benign and malignant tumors were 0.118min-1,0.257min-1,0.546,respectively.The Jorden index,sensitivity,specificity,positive predictive value,negative predictive values of the diagnosis of the ovarian benign and malignant tumors were 0.446,88.84％,57.23％,63.24％and 86.12％,respectively.The Jorden index,sensitivity, specificity,positive predictive value ,negative predictive values of Kep in the diagnosis of ovarian benign and malignant tumors were 0.804,88.91％,90.87％,89.15％and 90.78％,respectively.The Jorden index,sensitivity,specificity, positive predictive value ,negative predictive values of Ktrans in the diagnosis of ovarian benign and malignant tumors were 1.000,100.0％,100.0％,100.0％,100.0％.Conclusion Dynamic contrast -enhanced magnetic resonance imaging has differential diagnosis value on benign and malignant ovarian tumors .The ovarian benign tumors are mainly type Ⅰcurve,and the ovarian malignant tumors is mainly type Ⅲcurve.Ktrans is the best for differential diagnosis of ovarian benign and malignant tumors .
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Objective:To explore the role and mechanism of the B7-H1 expressed by auto-keratinocytes in the intermingled skin grafting model in vitro(MELC). Methods:The intermingled skin grafting model(MELC) was established in vitro. Flow cytometry was performed to detect the expressions of B7-H1 in keratinocytes. The expressions of PD-1 in lymphocytes were measured at the same time. The levels of IL-10,Foxp3 and GATA-3 mRNA in lymphocytes were detected by Real-time quantitative PCR. Results: Through flow eytometry,in the MELC with auto-keratinocytes,the expression of B7-H1 in auto-keratinocytes and the PD-1 in lymphocytes were rising trend and the rising rate was in time-dependent manners(P<0. 01). RT-PCR assay indicated that the relative levels of IL-10, Foxp3,GATA-3mRNA expression were significant raised and the rising rate was in time-dependent manners (P<0. 01). Conclusion:In the intermingled skin grafting model,the auto-keratinocytes could express B7-H1 to enhance the expression of PD-1 in T cells. When B7-H1 combined with PD-1,the Th2 cells and Foxp3+Tregs were induced and suppressed the immune response in the intermingled skin grafting model.
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Objective To compare the clinical value of dynamic enhanced CT,MRI in differential diagnosis of adrenal tumors.Methods 62 patients with adrenal tumor by pathologically confirmed in our hospital were select-ed.All patients underwent unenhanced and dynamic enhanced CT,MRI examination.The qualitative and adrenal tumor localization diagnostic accuracy,the adrenal adenomas and non adenoma tumor clearance rate of the two meth-ods were compared.Results The accuracy of enhanced CT positioning,qualitative examination were 96.77%, 90.57%,respectively,which were slightly higher than those of the MRI enhancement(91.94%,86.79%),but the differences were not statistically significant (χ2 =0.479,0.376,all P >0.05).53 adrenal adenomas and 9 cases non-adenomas absolute and relative clearance rate clearance rate in enhanced CT 5 min delay time,the differences were statistically significant (t =2.962,2.018,all P <0.05).53 cases adrenal adenomas and 9 cases non adenoma clear-ance of correction in the enhanced MRI delay 5min,respectively (33.41 ±20.17)%,(14.74 ±12.16)%,the differ-ence was statistically significant (t =2.684,P <0.05).Conclusion Dynamic enhanced CT,MRI are good methods for differential diagnosis of adrenal tumors,two methods have location and qualitative diagnosis correct rate.So dynamic enhanced CT,MRI are reliable means of diagnosis of adrenal tumors.
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Objective To explore the efficacy and safety of different doses of budesonide mixed suspension atomization inhalation in children with post infectious cough,and provide reference for the rational use of budesonide.Methods One hundred and twenty children with post infectious cough aged 2-14 years old were selected and divided into three groups by random digits table method (40 cases in each group).Control group was treated with 4 mg once montelukast sodium chewable tablets for 2-5 years old children,or 5 mg once for 6-14 years old children.Low-dose group was treated 2 times/d with a dose of 0.5 mg budesonide mixed suspension diluted in normal saline to 4 ml based on control group and high-dose group was treated 2 times/d with a dose of 1.0 mg budesonide mixed suspension diluted in normal saline to 4 ml based on control group.The outcome of curative effects were recorded and analyzed after 7 days of treatment.Results The total efficacy rate was 70.0％(28/40),52.5％(21/40),35.0％(14/40) in high-dose group,low-dose group and control group after 3 days of treatment,and there was significant difference between high-dose group and control group (P＜ 0.01).The total efficacy rate was 92.5％(37/40),90.0％(36/40),72.5％ (29/40) in high-dose group,low-dose group and control group after 7 days of treatment,and there was no significant difference(P ＞ 0.025).Few adverse events associated with inhaled corticosteroids,such as thrush,sore throat,hoarseness were observed in three groups.Conclusions Budesonide mixed suspension atomization inhalation alleviates symptom of post infectious cough significantly with no obvious side effects,even in high-dose group which has a more outstanding efficacy.Therefore,this therapy is valuable in clinical application.
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Objective To compare the therapeutic effect in two kinds of inhalation in infant asthmatic bronchitis.Methods Two hundred and twelve asthmatic bronchitis children were randomly divided into group A (103 cases) and group B (109 cases).The children in two groups were treated with conventional therapy,including antibiotics,fluids and other treatment.The children in group A were given budesonide 1 mg,terbutaline 5 mg plus 0.9％ sodium chlorine in 2 ml,with oxygen driven inhalation therapy,2-4 times/d.The children in group B were given albuterol sulfate inhalation aerosol 1-4 spray/times,4-6 times/d,inhaled fluticasone quantitative aerosol spray 1-2 spray/times,2 times/d,15 s/times.The course of two groups was 5-7 d.The therapeutic effect was compared.Results The total effective rate in group A and group B were 92.2 ％ (95/103) and 97.2 ％ (106/109),there was no significant difference (x2 =2.73,P ＞ 0.05).The relieve time of cough,gasping and wheezing,hospital stay in group B were shorter than those in group A [(12.00 ± 2.75) h vs.(29.00 ± 1.25) h,(10.00 ± 1.45) h vs.(31.00 ± 2.75) h,(13.00 ± 1.55) h vs.(33.00 ± 2.33) h,(7.20 ± 0.65) d vs.(8.70 ± 1.35) d],the cost of hospitalization in group B was lower than that in group A [(2.00 ± 0.30) thousand Yuan vs.(2.50 ± 0.55) thousand Yuan],there were significant differences(P ＜ 0.05).Conclusions The albuterol inhalation solution combined with fluticasone has the advantages of rapid efficacy,short course,low cost,easy to accept and adjust.It is worth to spread in primary hospital.
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Objective To investigate the effects of physical exercises on cardiac function and plasma N-terminal pro-brain natriuretic peptide (NT-proBNP) levels in hypertensive patients combined with diastolic cardiac dysfunction.MethodsA total of 66 essential hypertension patients who had abnormal left ventricular relaxation and normal systolic function were assigned to the intervention group ( n =33 ; doing physical exercises once a day,5 days a week) or control group (n =33 ).All the patients received standard treatment.At 6 months,body weight,blood pressure,heart rate,NT-proBNP,and echocardiography were measured.ResultsAt 6 months,body weight [ (68 ± 7 ) kg vs (72 ± 8 ) kg ],systolic blood pressure [ (135.4 ±5.1) mm Hg (1 mm Hg =0.133 kPa) vs (141.9 ±5.2) mm Hg ],diastolic blood pressure [ (81.1 ±4.0) mm Hg vs (84.7 ±4.6) mm Hg],New York Heart Association class (1.4 ±0.3 vs 1.8 ±0.4),NT-proBNP level [ (526 ± 126 ) ng/L vs (741 ± 189 ) ng/L] were significantly decreased in the intervention group when compared with the control group ( all P ＜ 0.05 ) although left ventricular ejection fraction (LVEF) (62.9 ±6.7 vs 59.0 ±5.6) and E/A ratio ( 1.1 ±0.3 vs 0.9 ±0.3) were significantly increased ( both P ＜ 0.05).ConclusionPhysical exercises could play a role in reduced blood pressure and body weight and improved cardiac function in hypertensive patients with diastolic cardiac dysfunction.
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Objective To assess the influence of community intervention on blood pressure and quality of life in aged patients with hypertension.Methods A total of 378 elderly hypertensive patients received community intervention with anti-high blood pressure agents for one year.Before and after intervention,blood pressure was recorded and genetic quality of life inventory(GQOLI-74),symptom checklist(SCL-90),self-rating anxiety scale(SAS),and self-rating depression scale(SDS)were carried out on the basis of giving unite guiding words.Results In addition to blood pressure degraded,the blood pressure control rate was improved after intervention(67.7% vs.40.1% before intervention,x2=57.59, P<0.05).Observation items of body health dimension,psychological health dimension,social function dimension were better than those recorded before intervention(P<0.05).But difference of the material life dimension was not statistically significant(P>0.05).With 41 scores as a SDS rude mark to judge the depression incidence.the incidence was decreased from 22.7% before intervention to 12.5%after intervention(x2=12.565,P<0.05).Conclusions Community intervention is a practical and valuable strategy for controlling high blood pressure and improving quality of life in aged patients.
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Objective To study the efficiency of reversing MDR by suppressing MDR1 gene with RNAi in K562/ADM cells. Methods For reversing MDR by RNAi technology, two different short hairpin RNAs (shRNAs) were designed and constructed into pGenSil-1 plasmid, respectively. They were then transfected into the highly adriamycin-resistant K562/ADM cell line. The RNAi effect on MDR was evaluated by real-time PCR, and Rhodamine123 (Rh123) efflux assy. Results The stable transfected clones showed varied degrees of reversal of MDR phenotype. Surprisingly, the MDR phenotype was completely reversed in two transfected clones. Conclusion This study demonstrates that MDR can be reversed by the shRNA-mediated RNAi in K562/ADM cells, which provides a valuable clue as to sensitizing multidrug-resistant hepatoma cells to anti-cancer drugs.
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Objective To stuay CT findings of adrenal gland trauma(AGT).Methods The CT images of adrenal gland trauma which were found in 1712 patients who had emergency abdominal CT examinations for assessment of blunt force trauma were analyzed restrospectively.Results Among 1712 cases.we identified 29 patients(1.7%)with adrenal gland traumatic lesions.A total of 32 lesions were found.Twenty-six lesions were unilateral(23 right-sided,3 left-sided),and 3 cases were bilateral(6 lesions).Main CT findings were as followed:(1)Adrenal hematomas appeared as discrete round or oval masses expanding the adrenal gland in 22 patients with 25 lesions.The lesions were hyperdense or of homogeneous density in plain scan and did not enhance in three-phase contrast-enhanced dynamic scan.The splayed adrenal limbs around hematoma were enhanced significantly as eurvilinear structures.(2)Mild to moderate uniform swelling of gland was shown in 4 cases.(3)Diffuse irregular hemorrhage obliterating the gland was shown in 3 cases.Contrast extravasation was shown in 1 case,which was active bleeding from the broken adrenal gland proven by operation.Associated CT findings included strand-like hemorrhage of the periadrenal fat and posterior pararenal hemorrhage mimicking thickened diaphragmatic crus.The concurrent injuries of ipsilateral thorax or/and abdomen were found in 25 patients.Conclusion The AGT has typical CT findings.Familiarity with characteristic CT findings of adrenal trauma is essential for the radiologist to avoid misdiagnosis.
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BACKGROUND: Bone marrow stromal cells(BMSCs) are the ideal gene target cells and will have a bright future in the gene therapy of spinal cord injury.OBJECTIVE :To detect the expression of glial cell line - derived neurotrophic factor(GDNF) gene after BMSCs were infected by adenovirus-medialed GDNF (Adv-GDNF) in vitro and to explore its biological activity.DESIGN: A randomized controlled trial study.SETTING: Laboratory of Orthopedic DepartmentMATERIALS: The experiment was completed in the Laboratory of Orthopedic Department, Affiliated Tongji Hospital of Tong ji Meidcal College,Huazhong University of Science and Technology. Twenty-four SD rats of either gender, weighing (180 ± 20) g.INTERVENTIONS: BMSCs were infected by Adv-GDNF in vitro and then cocultured with spinal cord dorsal root ganglion. The three methods, immunofluorescent chemistry, reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay(ELISA) were used to evaluate GDNF expression in the BMSCs. The biological activity of GDNF was observed by a phase contrast microscope.MAIN OUTCOME MEASURES:Primary outcomes:①RT-PCR;②results of immunofluorescent chemical examination;③biological activity of GDNF in vitro. Secondary outcomes:①culturing and identification of BMSCs②time-effect relationship of GDNF expression revealed by ELISA.RESULTS: Immunofluorescence displayed expression of GDNF in BMSCs 48hours after Adv-GNDF infection. RT-PCR analysis demonstrated expression of GDNF mRNA 24 hours after Adv-GNDF infection. ELISA confirmed the presence of GDNF in the liquid supernatant of BMSCs 24 hours after Adv-GDNF infectionn and showed that GDNF was secreted. The supernatant can promote the neurite outgrowth in the rat dorsal root ganglion(DRG).CONCLUSION: It is demonstrated that BMSCs infected by Adv-GDNF can express GDNF steadily and the expressed GDNF has the activity of promoting neurite outgrowth, which lays a foundation of the GDNF gene therapy for spinal cord injury.